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KMID : 0380020140290050328
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Korean Journal of Biotechnology and Bioengineering
2014 Volume.29 No. 5 p.328 ~ p.335
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Uptake of Fibroin Microspheres by 3T3 Cells
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Lee Jin-Sil
Go Nam-Kyung
Lee Shin-Young
Hur Won
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Abstract
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Vehicle toxicity is one of the main obstacles for intracellular delivery of bioactive compounds. Silk fibroin is a natural polymer proven to have high biocompatibility since being used as suture material. In this report, fibroin microspheres were prepared without any chemical modification or cross-linking not to affect its biocompatibility. The microspheres were taken up by more than 90% of 3T3 cells. Cellular uptake continued after medium replenishment with a different-colored fluorescent microsphere, suggesting that simultaneous ingestion and exocytosis occurred. Cellular uptake of fibroin microspheres did not affect cell viability. Intracellular trafficking of the microspheres using lysosome-specific fluorescent dye revealed that fibroin microspheres were localized both in the cytoplasm and in the lysosome. Accordingly, fibroin microspheres can be a potential vehicle for intracytoplasmic delivery of large cargos, such as mixtures of proteins, nutrients or artificial organelles.
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KEYWORD
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Fibroin microsphere, Cellular uptake, Intracellular delivery, 3T3 cell
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